Review



non effective 29 mer scrambled shrna cassette  (OriGene)


Bioz Verified Symbol OriGene is a verified supplier
Bioz Manufacturer Symbol OriGene manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    OriGene non effective 29 mer scrambled shrna cassette
    Non Effective 29 Mer Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+effective+29+mer+scrambled+shrna+cassette/Scrambled+shRNA+control+in+pRS+shRNA+Vector/bio_rxiv__2025__08__14__670350-272-8-19
    Average 95 stars, based on 176 article reviews
    non effective 29 mer scrambled shrna cassette - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Negative Control:

    Article Title: Mitofusin 2 Integrates Mitochondrial Network Remodelling, Mitophagy and the Renewal of Respiratory Chain Proteins in Neurons After Oxygen and Glucose Deprivation.
    Article Snippet: .. As a negative control, a non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector was used (scrRNA; Origene). ..

    shRNA:

    Article Title: Mitofusin 2 Integrates Mitochondrial Network Remodelling, Mitophagy and the Renewal of Respiratory Chain Proteins in Neurons After Oxygen and Glucose Deprivation.
    Article Snippet: .. As a negative control, a non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector was used (scrRNA; Origene). ..

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1×106 cells were transduced with 1μg of a green uorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25μg/ml puromycin (ant-pr-1, InvivoGen) for 4 weeks.

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1 × 10 6 cells were transduced with 1 μg of a green fluorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25 μg/ml puromycin (InvivoGen) for 4 weeks.

    Article Title: Laminin α1 orchestrates VEGFA functions in the ecosystem of colorectal carcinoma.
    Article Snippet: .. Generation of LMα1 knock-down cells HEK293T cells (ATCC CRL-3216; cultured in DMEM, 10% FCS, 1% penicillin-streptomycin) were transfected with pGFP-C-sh LAMA1 Lentivector (TL311806D: 5’- GAGATGTGCAGATGGTTACTATGGAAACC-3’) or pGFP-C-sh control Lentivector (TR30021) containing non-effective 29-mer scrambled shRNA cassette (OriGene, Cliniscience, Nanterre, France) together with pLP1, pLP2 and pLP/VSVG lentiviral packaging plasmids (Invitrogen, Life Technologies, Saint Aubin, France) to obtain lentiviral particles. ..

    Article Title: Src regulates amino acid-mediated mTORC1 activation by disrupting GATOR1-Rag GTPase interaction.
    Article Snippet: .. Scrambled: Non-effective 29-mer scrambled shRNA cassette (OriGene, TR30015) shRNAs targeting components of GATOR1 complex were purchased from CellBased Assay Screening Service (C-BASS) in Baylor College of Medicine. ..

    Article Title: Reconstructing the Ischemic Cerebrum by Transplanted Human Neurons
    Article Snippet: .. Human Ctip2 (BCL11B) 29mer shRNA plasmids (pRS-hU6-Ctip2shRNA-GFP-SV40-Puro) and non-effective 29-mer scrambled shRNA cassette in pRS Vector were obtained from OriGene (CAT#: TR306424). ..

    Article Title: Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons
    Article Snippet: .. Mouse, 4 unique 29-mer shRNA constructs against KIF3B in a lentiviral GFP vector (TL501182) with a non-effective 29-mer scrambled shRNA cassette in a pGFP-C-shLenti vector as control (TR30021) were obtained from OriGene. .. For knockdown experiments, cortical neurons [13–15 days in vitro (DIV)] were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions and allowed to express for 72 h. For KIF3B overexpression, a custom full-length KIF3B-GFP tagged plasmid (EX-Mm33992-Lv103, Genecopoeia) was generated.

    Transduction:

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1×106 cells were transduced with 1μg of a green uorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25μg/ml puromycin (ant-pr-1, InvivoGen) for 4 weeks.

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1 × 10 6 cells were transduced with 1 μg of a green fluorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25 μg/ml puromycin (InvivoGen) for 4 weeks.

    Plasmid Preparation:

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1×106 cells were transduced with 1μg of a green uorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25μg/ml puromycin (ant-pr-1, InvivoGen) for 4 weeks.

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1 × 10 6 cells were transduced with 1 μg of a green fluorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25 μg/ml puromycin (InvivoGen) for 4 weeks.

    Fluorescence:

    Article Title: Mitochondrial ROS drive resistance to chemotherapy and immune-killing in hypoxic non-small cell lung cancer
    Article Snippet: .. For silencing, 1 × 10 6 cells were transduced with 1 μg of a green fluorescence protein (GFP)-lentiviral plasmid containing a non-effective 29-mer scrambled shRNA cassette (Origene, Rockville, MD), two different sequences targeting HIF-1α (TL320380, Origene), two different sequences targeting C/EBP-β (TL320301, Origene). .. Stably silenced clones were generated by selecting cells with 0.25 μg/ml puromycin (InvivoGen) for 4 weeks.

    Knockdown:

    Article Title: Laminin α1 orchestrates VEGFA functions in the ecosystem of colorectal carcinoma.
    Article Snippet: .. Generation of LMα1 knock-down cells HEK293T cells (ATCC CRL-3216; cultured in DMEM, 10% FCS, 1% penicillin-streptomycin) were transfected with pGFP-C-sh LAMA1 Lentivector (TL311806D: 5’- GAGATGTGCAGATGGTTACTATGGAAACC-3’) or pGFP-C-sh control Lentivector (TR30021) containing non-effective 29-mer scrambled shRNA cassette (OriGene, Cliniscience, Nanterre, France) together with pLP1, pLP2 and pLP/VSVG lentiviral packaging plasmids (Invitrogen, Life Technologies, Saint Aubin, France) to obtain lentiviral particles. ..

    Cell Culture:

    Article Title: Laminin α1 orchestrates VEGFA functions in the ecosystem of colorectal carcinoma.
    Article Snippet: .. Generation of LMα1 knock-down cells HEK293T cells (ATCC CRL-3216; cultured in DMEM, 10% FCS, 1% penicillin-streptomycin) were transfected with pGFP-C-sh LAMA1 Lentivector (TL311806D: 5’- GAGATGTGCAGATGGTTACTATGGAAACC-3’) or pGFP-C-sh control Lentivector (TR30021) containing non-effective 29-mer scrambled shRNA cassette (OriGene, Cliniscience, Nanterre, France) together with pLP1, pLP2 and pLP/VSVG lentiviral packaging plasmids (Invitrogen, Life Technologies, Saint Aubin, France) to obtain lentiviral particles. ..

    Transfection:

    Article Title: Laminin α1 orchestrates VEGFA functions in the ecosystem of colorectal carcinoma.
    Article Snippet: .. Generation of LMα1 knock-down cells HEK293T cells (ATCC CRL-3216; cultured in DMEM, 10% FCS, 1% penicillin-streptomycin) were transfected with pGFP-C-sh LAMA1 Lentivector (TL311806D: 5’- GAGATGTGCAGATGGTTACTATGGAAACC-3’) or pGFP-C-sh control Lentivector (TR30021) containing non-effective 29-mer scrambled shRNA cassette (OriGene, Cliniscience, Nanterre, France) together with pLP1, pLP2 and pLP/VSVG lentiviral packaging plasmids (Invitrogen, Life Technologies, Saint Aubin, France) to obtain lentiviral particles. ..

    Control:

    Article Title: Laminin α1 orchestrates VEGFA functions in the ecosystem of colorectal carcinoma.
    Article Snippet: .. Generation of LMα1 knock-down cells HEK293T cells (ATCC CRL-3216; cultured in DMEM, 10% FCS, 1% penicillin-streptomycin) were transfected with pGFP-C-sh LAMA1 Lentivector (TL311806D: 5’- GAGATGTGCAGATGGTTACTATGGAAACC-3’) or pGFP-C-sh control Lentivector (TR30021) containing non-effective 29-mer scrambled shRNA cassette (OriGene, Cliniscience, Nanterre, France) together with pLP1, pLP2 and pLP/VSVG lentiviral packaging plasmids (Invitrogen, Life Technologies, Saint Aubin, France) to obtain lentiviral particles. ..

    Article Title: Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons
    Article Snippet: .. Mouse, 4 unique 29-mer shRNA constructs against KIF3B in a lentiviral GFP vector (TL501182) with a non-effective 29-mer scrambled shRNA cassette in a pGFP-C-shLenti vector as control (TR30021) were obtained from OriGene. .. For knockdown experiments, cortical neurons [13–15 days in vitro (DIV)] were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions and allowed to express for 72 h. For KIF3B overexpression, a custom full-length KIF3B-GFP tagged plasmid (EX-Mm33992-Lv103, Genecopoeia) was generated.

    other:

    Article Title: C/EBP-β LAP regulates chemo-immuno-resistance in hypoxic non-small cell lung cancer
    Article Snippet: LAP and LIP induction was activated by adding 1μg/ml doxycycline (D9891, Sigma Aldrich) in the culture medium.

    Construct:

    Article Title: Molecular Motor KIF3B Acts as a Key Regulator of Dendritic Architecture in Cortical Neurons
    Article Snippet: .. Mouse, 4 unique 29-mer shRNA constructs against KIF3B in a lentiviral GFP vector (TL501182) with a non-effective 29-mer scrambled shRNA cassette in a pGFP-C-shLenti vector as control (TR30021) were obtained from OriGene. .. For knockdown experiments, cortical neurons [13–15 days in vitro (DIV)] were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions and allowed to express for 72 h. For KIF3B overexpression, a custom full-length KIF3B-GFP tagged plasmid (EX-Mm33992-Lv103, Genecopoeia) was generated.



    Similar Products

    95
    OriGene non effective 29 mer scrambled shrna cassette
    Non Effective 29 Mer Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+effective+29+mer+scrambled+shrna+cassette/Scrambled+shRNA+control+in+pRS+shRNA+Vector/bio_rxiv__2025__08__14__670350-272-8-19
    Average 95 stars, based on 1 article reviews
    non effective 29 mer scrambled shrna cassette - by Bioz Stars, 2026-11
    95/100 stars
      Buy from Supplier

    93
    OriGene mer non effective scrambled shrna cassette
    Mer Non Effective Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+effective+29+mer+scrambled+shrna+cassette/Glucose+6+Phosphate+Dehydrogenase+(G6PD)+Human+shRNA+Plasmid+Kit/pm39772392-267-14-23
    Average 93 stars, based on 1 article reviews
    mer non effective scrambled shrna cassette - by Bioz Stars, 2026-11
    93/100 stars
      Buy from Supplier

    91
    OriGene effective 29 mer scrambled shrna cassette
    Effective 29 Mer Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+effective+29+mer+scrambled+shrna+cassette/CCR5+Human+shRNA+Plasmid+Kit/pmc10667812-203-7-18
    Average 91 stars, based on 1 article reviews
    effective 29 mer scrambled shrna cassette - by Bioz Stars, 2026-11
    91/100 stars
      Buy from Supplier

    90
    OriGene gfp-lentiviral plasmid containing a non-effective 29-mer scrambled shrna cassette
    Gfp Lentiviral Plasmid Containing A Non Effective 29 Mer Scrambled Shrna Cassette, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+effective+29+mer+scrambled+shrna+cassette/scrambled+shrna+origene+tr30015/pm35953814-135-13-25
    Average 90 stars, based on 1 article reviews
    gfp-lentiviral plasmid containing a non-effective 29-mer scrambled shrna cassette - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    Image Search Results